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Tuesday, July 11, 2006

A replacement for PCR
Here's a must read from PLoS. It's essentially a replacement for PCR using a process called recombinase polymerase amplification.

RPA uses five main ingredients: a sample of the DNA to be amplified; a primer–recombinase complex, which initiates the copying process when it attaches to the template; nucleotides from which to form the new strands; a polymerase, which brings them together in the right order; and single-stranded DNA-binding proteins (SSBs), which help keep the original DNA from zipping back together while the new DNA is being made. The primer–recombinase complex is able to attach to the double-stranded DNA, eliminating the need to heat the mixture. After the complex is in place, it disassembles, allowing the DNA polymerase to begin synthesizing a new strand of DNA complementary to the template, while the SSBs attach to and stabilize the displaced strand. Under the right conditions—a precise milieu of process-regulating chemicals—the process automatically repeats, resulting in an exponential increase in the DNA sample.


The reason this process is so great is that it wouldn't require any specialized equipment for the amplification, unlike PCR which requires a thermal cycler and a prolonged process of heating and cooling the sample to amplify the target. Now these researchers have developed a method that could perform the same job at a constant low temperature, allowing the possibility for field PCR testing and impressively, created a dipstick test for rapid identification of MRSA without any specialized machinery.

Kickass!

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